Arsenic Trioxide Reduces Global Histone H4 Acetylation at Lysine 16 through Direct Binding to Histone Acetyltransferase hMOF in Human Cells
Fig 3
As2O3 can bind directly to hMOF.
(A) Schematic of As-immobilized agarose preparation. (B) As-immobilized agarose pulled down hMOF. (C&D) Competitive inhibition between As and hMOF. H2B protein is as negative control. Western blot images were scanned and signals were densitometrically quantified with Quantity One Basic software (Bio-Rad). The percentage of bound hMOF in the flow through appears in D.