The Effector Domain of MARCKS Is a Nuclear Localization Signal that Regulates Cellular PIP2 Levels and Nuclear PIP2 Localization
Fig 4
Changes in PIP2 in ED-deleted MARCKS expressing glioma cells.
A) Nuclear PIP2 immunofluorescence staining was quantified in the U87 WT- and ΔED- MARCKS expressing cells as described in Materials and Methods. Briefly, DAPI-defined nuclear regions of interest were generated and an integrated density value (IDV) was calculated for each nucleus. The mean IDV and standard error are shown (t-test p = 0.0043). B) Total levels of PIP2 were quantified for U87 WT- and ΔED- MARCKS expressing cells with or without doxycycline treatment using a PI(4,5)P2 Mass ELISA KIT. Mean PIP2 concentrations with standard errors are shown (t-test p< 0.0001).