Downhill Running-Based Overtraining Protocol Improves Hepatic Insulin Signaling Pathway without Concomitant Decrease of Inflammatory Proteins
Fig 3
Ratio between pIRbeta (Tyr)/IRbeta (arbitrary units) (A), ratio between pIRS–1 (Ser307)/pIRS–1 (arbitrary units) (B), ratio between pAkt (Ser473)/Akt (arbitrary units) (C), ratio between pGSK3beta (Ser9)/GSK3beta (arbitrary units) (D), and ratio between pFoxo1 (Ser256)/Foxo1 (arbitrary units) (E), and liver glycogen concentrations (mg.100mg−1of tissue) (F) for the experimental groups. The blot for IRbeta was performed on total protein liver extracts. Data correspond to means ± SE of n = 6 mice. CT: sedentary mice; OTR/down: overtrained by downhill running; OTR/up: overtrained by uphill running; OTR: overtrained by running without inclination.*P < 0.05 vs. CT. †P < 0.05 vs. OTR/down. **P < 0.05) vs. OTR/up. ††P < 0.05 vs. OTR.