A JAZ Protein in Astragalus sinicus Interacts with a Leghemoglobin through the TIFY Domain and Is Involved in Nodule Development and Nitrogen Fixation
Fig 6
Symbiotic phenotypes associated with AsJAZ1-RNAi.
Hairy roots and nodules with empty pCAMBIA1301-35S-int-T7 served as the control samples. The phenotype was observed 30 days after inoculation. (A) Efficiency of RNAi in hairy roots. The experiment was performed in triplicate. Nine to 10 hairy root plants were analyzed in each experiment and the total numbers of control and RNAi plantlets were 30 and 28, respectively. Bars represent the SE of three independent experiments. **, difference is significant at P<0.01. (B) Above-ground sections for control (left, CK1301) and RNAi hairy roots. (C) Appearances of control (left 3) and RNAi (right 3) hairy root plants. (D) Appearance of root nodules on control (left, CK1301) and RNAi hairy roots. The arrows indicate normal nodules on control roots or ineffective nodules on RNAi hairy roots. (E) Number of nodules on control and RNAi hairy roots. The experiment was performed in triplicate and bars represent the SE of three independent experiments. **, difference is significant at P < 0.01.