Synergistic Gene Expression Signature Observed in TK6 Cells upon Co-Exposure to UVC-Irradiation and Protein Kinase C-Activating Tumor Promoters
Fig 2
Synergistic gene expression in response to TPA+UVC treatment.
(A) Compared to the non-irradiated vehicle control, there were more DEGs (fold-change ±2 and FDR<0.05) in the TPA+UVC treated cells compared to UVC or TPA alone. The total number of DEGs was higher than the theoretical sum of TPA and UVC alone and was sustained through 24 hours, representing a synergistic effect. (B) UVC caused mostly down-regulation early which was reversed by 24 hours. TPA+UVC caused a similar trend with a greater amount of DEGs at each time-point. TPA, in contrast, caused similar levels of up and down-regulation at each time point. Therefore, UVC treatment was more influential on overall gene expression patterns in the co-treated cells compared to TPA.