The Urine Proteome Profile Is Different in Neuromyelitis Optica Compared to Multiple Sclerosis: A Clinical Proteome Study
Fig 3
False Discovery Rate Adjustment identifies proteins significant for NMO/NMO-SD and HS discrimination in the urine.
(A) Heat maps comparing NMO/NMO-SD and HS samples by false discovery rate adjustment with q-values less than 0.05 are shown. The analysis identified 31 proteins that discriminated NMO/NMO-SD from HS. (B) Only 3 fragments of Igs appeared to be upregulated compared to HS, the rest of the proteins were downregulated. (C) ROC curves for the Ig chains Ig-G3, Ig-K and Ig-L are shown. (D) Detection of Ig light chains by LC-MS/MS was 100% in Cohort 1, while only 20–30% by nephelometry. In cohort 2, nephelometry detected Ig light chains only in 10% of NMO/NMO-SD samples. Magenta, upregulated compared to HS; Green, downregulated compared to HS; NMO/NMO-SD, neuromyelitis optica/neuromyelitis optica spectrum disorder; MS, multiple sclerosis; HS, healthy subjects; Ig-G3, immunoglobulin gamma–3 chain; Ig-K; immunoglobulin kappa chain; Ig-L, immunoglobulin lambda chain.