Small RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat Grains
Fig 5
Identification of two unique targets for non-conserved development-related miRNAs.
(A) A target (TC453857) was regulated by two different miRNAs. The predicted cleavage sites (C1 and C2) were shown in the blue and red boxes in the transcript, respectively. The blue or red lines in the t-plot indicate sequence abundances consistent with the C1 site or C2 site. miRNA:mRNA alignments along with C1 and C2 sites are shown above. (B) A target (TC389301) with multiple similar miRNA sites was intensively regulated by miR9666. The predicted cleavage sites are shown as red boxes in the transcript. The red lines in the t-plot indicate sequence abundances consistent with the C1–C4 sites. miRNA:mRNA alignments along with C1–C4 sites are shown above. Degradome data origins are indicated in the t-plots.