Changes in the Sterol Composition of the Plasma Membrane Affect Membrane Potential, Salt Tolerance and the Activity of Multidrug Resistance Pumps in Saccharomyces cerevisiae
Fig 5
Activity of Pdr5 and Snq2 pumps.
Changes in diS-C3(3) staining of (A) wild-type BY4741 (black circles), erg5Δ (blue diamonds), pdr5Δ (pink squares), snq2Δ (violet hexagons) and (B) erg2Δ (red squares), erg3Δ (green triangles), erg4Δ (yellow triangles) and erg6Δ (cyan hexagons) cells after addition of 10 μM CCCP (full symbols); control (empty symbols). (C) Changes in diS-C3(3) staining of wild-type cells (grey and black symbols) after addition of CCCP or FK506: 5 μM CCCP (diamonds), 10 μM CCCP (squares), 20 μM CCCP (inverted triangles), 10 μM FK506 (hexagons), 100 μM FK506 (triangles), control (circles) in comparison with staining of pdr5Δ (pink symbols) and snq2Δ cells (violet symbols): 10 μM CCCP (squares), 10 μM FK506 (hexagons), control (circles). (D) Changes in diS-C3(3) staining of wild-type (black circles), erg2Δ (red squares), erg3Δ (green triangles), erg4Δ (yellow triangles), erg5Δ (blue diamonds), erg6Δ (cyan hexagons), pdr5Δ (pink squares) and snq2Δ (violet hexagons) cells after addition of 100 μM FK506 (full symbols); control (empty symbols). Arrows with dotted lines indicate addition of compounds. (E) Growth inhibition zones of cells exposed to 30 mM FK506, 6.5 mM fluconazole, 5 mM NQO and their combinations.