Proteomics of Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles
Fig 1
Purification of OMVs from A. actinomycetemcomitans strain 173.
(A) Atomic force micrograph of OMVs obtained from strain 173 after density gradient centrifugation. Bar = 300 nm. (B) Reactivity of the isolated strain 173 OMVs (lane 1) to a rabbit antiserum made against whole A. actinomycetemcomitans serotype e bacterial cells. OMVs from strain D7SS (serotype a) are loaded in lane 2. Samples equal to 10 μg protein were applied on the gel. (C) SDS-PAGE analysis of density gradient fractions of strain 173 OMVs. Fractions (15 μl loaded on the gel) are numbered according to increasing density. The central fractions (8–12) were pooled and subject to LC-MS/MS analysis. The sizes (kDa) of the proteins in the pre-stained molecular weight marker (M) are indicated along the left side of the gels in panel B, and C. The images show one representative experiment.