Effect of Creosote Bush-Derived NDGA on Expression of Genes Involved in Lipid Metabolism in Liver of High-Fructose Fed Rats: Relevance to NDGA Amelioration of Hypertriglyceridemia and Hepatic Steatosis
Fig 3
Effect of acute NDGA treatment on the expression of genes and transcription factors involved in hepatic fatty acid metabolism and development of hepatic steatosis in high-fructose diet (HFrD) fed hypertriglyceridemic rats.
Quantitative RT-PCR of RNA from livers of chow-fed rats, rats fed an HFrD for 8 weeks and rats fed an HFrD for 8 weeks and subsequently treated with NDGA at a dose of 125 mg/kg BW twice a day by oral gavage. Results are mean ± SE of 3 independent RT-PCR experiments for each gene with 6 rats within each group. Data are presented relative to 36B4 in the same samples, and relative mRNA levels were determined by qRT-PCR using the comparative CT method. * P<0.05; ** P<0.01; *** P<0.001. Raw data and statistics are listed in S1 Dataset.