In Situ Transplantation of Alginate Bioencapsulated Adipose Tissues Derived Stem Cells (ADSCs) via Hepatic Injection in a Mouse Model
Fig 4
Assessment of the in vivo fate of bioencapsulated ADSCs 3 weeks after in situ transplantation.
(A-D) Hepatogenic differentiation of bioencapsulated ADSCs: mouse liver stained by anti-hAAT antibody (A and C); Mouse liver stained by anti-albumin antibody (B and D), in which B is the consecutive liver section of A, and D is the consecutive liver section of C; (E) Validation of the specificity of anti-albumin antibody: mouse liver (after PH but without cell transplantation) stained by anti-albumin antibody; (F) Evaluation of the interaction between alginate and anti-hAAT antibody: mouse liver containing empty alginate microspheres stained by anti-hAAT antibody; (G) The proliferative activity of ADSCs within the residual alginate microspheres was detected by anti-ki67 antibody. The arrows indicated ki67 positive cells; (H) Evaluation of generation of anti-human AAT antibody by ELISA. Serum samples collected from normal C57BL/6 mice without any treatment were used as a negative control (NC). The dotted line indicated the lower limit of quantification (LLOQ) (N = 4).