Ischemic Preconditioning Mediates Neuroprotection against Ischemia in Mouse Hippocampal CA1 Neurons by Inducing Autophagy
Fig 5
The neuroprotective effects of IPC-induced autophagy in the hippocampal CA1 layer.
Representative images of Nissl staining of the hippocampus. Mice were exposed to 1 min × 3 sublethal BCCAO, a combination of IPC and intraventricular injection of 3-MA, or IPC and intraventricular injection of saline followed by 20 min lethal BCCAO 24 h later. Cell survival was then quantified by Nissl staining in hippocampal CA1 (A, B, D, E, G, H, J, K, M, and N) and CA3 (C, F, I, L, and O) layers 24 h later. Values are given as the mean number of neurons. Error bars denote SDs. *P < 0.01 compared with the control group. #P < 0.05 compared with the nonpreconditioning group. ☆P < 0.01 compared with the preconditioning group. ☆☆P > 0.05 compared with the preconditioning group. Original magnification, 2× (A, D, G, J, and M) and 10× (B, C, E, F, H, I, K, L, N, and O).