Histone Deacetylase 3 and 4 Complex Stimulates the Transcriptional Activity of the Mineralocorticoid Receptor
Fig 3
HDAC4 acts as a scaffold between the MR and HDAC3.
Treatment with Aldo resulted in increased expression of GILZ (A) and SGK-1 (B), which was decreased by knockdown of HDAC4. Graph shows the means ± SE of three independent experiments (*p < 0.05 vs. vehicle, #p<0.05 Scramble vs. siRNA). HDAC4 knockdown decreased the Aldo-induced recruitment of MR and Pol II to GILZ (C) and SGK-1 (D) promoters. Schematic diagrams show the locations of HRE and PCR amplification after chromatin immunoprecipitation (ChIP) in GILZ (C) and SGK-1 (D) promoters. Graph shows the means ± SE of three independent experiments (*p < 0.05 vs. vehicle, #p<0.05 Scramble vs. siRNA). E, HDAC4 protein was significantly decreased after 48 h of siRNA transfection. F, The interaction between MR and HDAC3 induced by Aldo was inhibited by knockdown of HDAC4. G, Acetylation of MR was increased by HDAC4 knockdown when HEK293 cells were stimulated by Aldo.