Type I Interferons Function as Autocrine and Paracrine Factors to Induce Autotaxin in Response to TLR Activation
Fig 8
Type I interferons induce ATX expression in human PBMCs and monocytes.
(A)Human PBMCs and monocytes were treated with IFN-α (50 ng/ml) for 2h or with IFN-β (10 ng/ml) for 4 h, and then ATX mRNA expression was detected by qRT-PCR. (B) HUVECs and Jurkat cells were treated with IFN-α (50 ng/ml) for 2 h or with IFN-β (10 ng/ml) for 4 h, and then ATX mRNA expression was detected by RT-PCR. (C, D)Human PBMCs and monocytes were pretreated with LY294002 (10 μM), SB202190 (10 μM), PD98059 (20 μM), or pyridone 6 (P6; 10 μM) for 30 min before the IFN-α/β treatment. After IFN-α treatment for 2 h or IFN-β treatment for 4 h, ATX mRNA levels were detected by qRT-PCR. The ATX expression detected by qRT-PCR analyses was normalized to expression of GAPDH and presented relative to expression in untreated cells. All qRT-PCR data are expressed as mean values ± SD, n = 3. The p values derived from Student’s t test are (*) p < 0.05, (**) p < 0.01. A representative experiment out of three is shown.