Inactivated Enterovirus 71 Vaccine Produced by 200-L Scale Serum-Free Microcarrier Bioreactor System Provides Cross-Protective Efficacy in Human SCARB2 Transgenic Mouse
Fig 4
T helper cell responses in vaccinated mice.
The levels of IL-4 and IFN-γ spot-forming cells in the spleens of mice were determined by ELISpot assay. Mice groups (n = 5) were immunized with PBS and various dosages of EV71 vaccines including AddaVAX-EV71, AlPO4-EV71, or EV71 antigen as indicated. Splenocytes from PBS- or vaccine-treated mice were counted and stimulated in vitro with heat-inactivated whole viruses of EV71(E59-B4), 5746 (C2), or 3340 (C4) strains. The cells were stimulated with ConA (1 μg/mL) as a positive control for the IFN-γ ELISpot assay or were mock stimulated as a negative control (w/o) for both ELISpot assays. Bars represent the means ± SEM of spot counts in triplicate wells. The p value between vaccine groups relative to the PBS control and two vaccine groups were labeled.