The UVB-Stimulated Expression of Transglutaminase 1 Is Mediated Predominantly via the NFκB Signaling Pathway: New Evidence of Its Significant Attenuation through the Specific Interruption of the p38/MSK1/NFκBp65 Ser276 Axis
Fig 11
Effects of AX on the Ser133 phosphorylation of CREB in UVB-exposed human HaCaT keratinocytes at 0.5 and 3 h min post-irradiation.
(A) Western blotting for the phosphorylation of CREB Ser133. (B) Densitometric analysis for Ser133 phosphorylation of CREB at 0.5 h post-irradiation. Human HaCaT keratinocytes were exposed to UVB irradiation at a dose of 80 mJ/cm2 immediately after which they were treated with AX at the indicated concentrations. Lysates were harvested at 0.5 and 3 h after UVB irradiation and were immunoblotted with antibodies to phosphorylated or non-phosphorylated CREB. Representative immunoblots from 3 independent experiments are shown; values are means ± S.D. from 3 independent experiments. **: p<0.01.