Male Sex is Associated with a Reduced Alveolar Epithelial Sodium Transport
Fig 7
Inhibition of ER-β abolishes the sex differences in Na+ transport and female FDLE cells are more responsive to E2 stimulation.
(A) Ibase and Iamil were significantly higher in FDLE cells of female origin, while PHTPP abolished the sex difference (n = 23–28; mean + SEM; *p<0.05; **p<0.01 by T-test). (B) Vbase and Vamil were significantly higher in FDLE cells of female origin, while PHTPP abolished the sex difference (n = 23–28; mean + SEM; *p<0.05 by T-test). (C) PHTPP decreased Rte, but no Rte differences were observed between male and female FDLE cells. (D) PDGF-A mRNA expression in response to different E2 concentrations in male and female FDLE monolayers. Female cells are more responsive to E2 since PDGF-A mRNA expression was significantly increased in females, whereas PDGF-A mRNA expression was not affected by E2 in male FDLE cells. (♂: relative difference to male control; ♀: relative difference to female control; n = 6; mean + SEM; *p<0.05 by ANOVA with Dunnett’s post hoc test).