Creation of Mice Bearing a Partial Duplication of HPRT Gene Marked with a GFP Gene and Detection of Revertant Cells In Situ as GFP-Positive Somatic Cells
Fig 2
Distributions of mutational events per 106 cells examined.
In the liver (A) and pancreas (B), frozen sections were scored under a fluorescent microscopy and a cluster of GFP-positive cells that were interrelated across slices were counted as a single event. In small intestine (C), green streaks that appeared in villi were scored using a dissecting microscopy. The results were used to estimate mutational events per 106 crypt stem cells (see Materials and Methods). In spleen (D), mutant cells were detected by a flow cytometer and the frequency was expressed per 106 cells. Open and filled circles represent animals receiving 0-Gy and 3-Gy irradiation, respectively. Triangles and squares represent means and medians, respectively. Asterisks (panel D) represent extreme outliers, with their actual values provided in the figure. No extreme jackpot mutations were detected in this series of the experiments.