Identification of the Mitochondrial Heme Metabolism Complex
Fig 1
Graphical representation of affinity purification and MS analysis of FLAG-FECH (red), FLAG-FECH Variants (purple), FLAG-PPOX (orange) and FLAG-CPOX (blue).
Each panel represents an identified mouse protein recovered with the bait human protein listed in the legend of panel A. Panels are as follows: (A)—fech, (B)—ppox, (C)—cpox and (D)—alas2, (E)—sucla2, (F)—abcb10 and (G)—abcb7. Number of unique peptides (x axis), % sequence coverage (y axis) and spectral counts (bubble size) for each was calculated using the maximal values obtained minus the maximal values observed in the control samples (empty vector). Size of bubbles represents the % of the total spectral counts identified for each mouse protein. The maximal spectral counts of each of the mouse proteins was fech = 971, ppox = 71, cpox = 256, alas-2 = 16, sucla2 = 16, abcb10 = 51 and abcb7 = 36. Note no mouse peptides for alas2, sucla2, or abcb10 were obtained using FLAG-CPOX as bait above that of empty vector.