Microsomal Triglyceride Transfer Protein (MTP) Associates with Cytosolic Lipid Droplets in 3T3-L1 Adipocytes
Fig 6
MTP is associated with isolated lipid droplets.
3T3-L1 cells were induced to differentiate and on day 6 lipid droplets were isolated as described in Experimental Procedures. Lipid droplets floating at the top of the discontinuous sucrose gradient were recovered (C, Control). Aliquots of this fraction were incubated with equal volumes of hypotonic lysis medium (Washed, W) or 0.2 M Na2CO3, pH 11.0, (Hi pH, H), re-floated through the discontinuous sucrose gradient, and recovered. All fractions were delipidated using -80°C acetone. The precipitated lipid droplet proteins were solubilized in SDS PAGE solubilizing buffer and analyzed by immunoblotting.