Generation of Naïve Bovine Induced Pluripotent Stem Cells Using PiggyBac Transposition of Doxycycline-Inducible Transcription Factors
Fig 6
Differentiation potential of biPSCs in culture.
(A) Embryoid body formation of pbiPSCs grown for 6 days in low cell-adhesion dishes. Immunocytochemical staining for markers for the three germ-layer in differentiated cells derived from pbiPSCs. α-fetoprotein (B, endoderm), actin smooth muscle (C, mesoderm), and glial fibrillary acidic protein (D, ectoderm) were used as markers. (E) Embryoid body formation by pnbiPSCs. Immunocytochemical staining for α-fetoprotein (F), actin smooth muscle (G), glial fibrillary acidic protein (H). (A), (E), scale bars = 500 μm. (B)–(D), (F)–(H), scale bars = 100 μm.