Promoter Hypomethylation of Maspin Inhibits Migration and Invasion of Extravillous Trophoblast Cells during Placentation
Fig 1
Effect of hypoxia on protein expression of maspin, apoptotic and invasive ability in TEV-1 A: The maspin protein in the normoxic and hypoxic groups expressed as a relative measure compared to GAPDH and normalized to a control sample run on each gel at two different culture time points (24h, 48h).
The protein expression of maspin in the HOX group was significantly higher compared to the NOX group at both 24h and 48h, the protein expression of maspin at 48h was significantly higher compared to 24h; (*P < 0.05). B: Representative Western blot analysis of maspin protein expression in the NOX and HOX groups; C: Apoptosis rate of cells was measured by flow cytometry. The apoptosis rate in the HOX group was significantly higher compared to the NOX group at 12h, 24h and 48h. (*P < 0.05) D: The invasive ability of cells were tested by transwell method. The invaded cells in the HOX group was significantly fewer compared to the NOX group at 48h. (*P < 0.05) E: Photo of invaded cells in the NOX and HOX group (200X).