Deficiency of Lipoprotein Lipase in Neurons Decreases AMPA Receptor Phosphorylation and Leads to Neurobehavioral Abnormalities in Mice
Fig 2
NEXLPL+/- mice have multiple neurobehavioral abnormalities.
(A) Motor activities were tested in an open field apparatus (CTR n = 9; NEXLPL+/- n = 12). (B) Spatial learning and memory was tested with Morris water maze at 21+ mo age: Day 1: visible platform; Day 4–8: hidden platform, learning phase; Day 11: hidden platform, short memory; Day13: platform position changed. From Day 4 to Day 8, one way repeated measure ANOVA showed genotype difference, p<0.01, (CTR n = 14; NEXLPL+/- n = 15). (C) Spatial learning and memory was tested with Morris Water Maze described above at 13 mo age (CTR n = 11; NEXLPL+/- n = 12). (D-E) Measurement of weights and swimming speeds of chow-fed CTR (n = 4), high fat (HF)-fed CTR (n = 6), and HF-fed NEXLPL+/- (n = 4) mice. (F) Recognition memory was tested with novel object recognition. The test is performed as 2 trials. In trial 1 (T1), two identical objects were placed in the apparatus, followed by trial 2 (T2) with replacement of one object. Discrimination ratio = (Tnew-Told)/(Tnew+Told) (CTR n = 9; NEXLPL+/- n = 10, p = 0.04). (G) Elevated plus maze: Inset is the representative tracts of mice. The apparatus was 50 cm above the ground with two open arms and two closed arms (CTR n = 9; NEXLPL+/- n = 11, p = 0.02).