Phosphotyrosine Substrate Sequence Motifs for Dual Specificity Phosphatases
Fig 1
(A) Coomassie blue staining of a SDS-PAGE gel showing the recombinant DUSP proteins examined. (B) Scanned images of DUSP treated Tyr(P) peptide microarrays. The human Tyr(P) peptides (>6000) were microarrayed in three identical subarrays on each slide. The microarrays were incubated with individual DUSPs and remaining Tyr(P) content was measured using anti- Tyr(P) monoclonal antibody and an Alexa-635 secondary (anti-mouse IgG) antibody. The control reference slide was treated with buffer only. The images were obtained from the same area of each slide. Each spot represents one peptide.