The Inhibitory Core of the Myostatin Prodomain: Its Interaction with Both Type I and II Membrane Receptors, and Potential to Treat Muscle Atrophy
Fig 8
Local injection of p29 alleviates the reduction in myofiber size by restoration of the decreased numbers of satellite cells.
(A) Histological analysis of TA muscles treated with p29 or albumin (control) in wild-type (upper) and CAV3P104L (lower) mice. Scale bar, 50 μm (left). Distribution of SMAs in TA muscles of mice treated with p29 or albumin (right; n = 7; 250 myofibers were assessed in each mouse). (B) Immunohistochemical analysis of M-cadherin-positive satellite cells (green, arrows) in TA muscles of wild-type (upper) and CAV3P104L (lower) mice (left) treated with p29 or albumin. Red indicates laminin α2 and gray indicates nuclei. Numbers of satellite cells per 100 myonuclei in TA muscles (right). One-thousand myonuclei were assessed in each muscle (n = 7). *P < 0.05. (C) Fluorescence images of satellite cells attached to single myofibers isolated from the TA muscles of wild-type (Wild) and CAV3P104L mice treated with (+) or without (–) p29 (left). Mouse caveolin 1 (CAV1) was used as a marker of satellite cells (green). Nuclei were counterstained with DAPI (blue). The white arrow indicates (satellite cells). Quantification of the number of satellite cells attached to single myofibers (right). Numbers of satellite cells per 100 myonuclei (right). Data are expressed as the mean ± SD (n = 5). *P < 0.05.