The Drosophila Transcription Factors Tinman and Pannier Activate and Collaborate with Myocyte Enhancer Factor-2 to Promote Heart Cell Fate
Fig 7
Expression of tinman, pannier and Mef2 in the ectoderm results in an expansion of cardiac gene expression.
(A,B) 69B>tin+pnr+Mef2 embryos at stage 14, stained for Hand expression (A) or Sur expression (B). (A) ~ 50% of embryos had expanded Hand expression (arrowhead) with the rest demonstrating normal expression in the heart (inset, arrow). (B) ~70% of embryos had normal Sur expression in the heart (arrow), with a subset showing expanded Sur expression in the nervous system (inset, arrowhead). Bar, 100μm. (C) Quantification of effects of over-expression of cardiac transcription factors. (D,E) Antibody stain against Fasciclin III. (D) shows normal FasIII accumulation in a stage 9 control embryo. (E) shows similar levels of accumulation in a 69B>tin+pnr+Mef2 embryo.