miR-34 and p53: New Insights into a Complex Functional Relationship
Fig 3
Overexpression of miR-34a-resistant SIRT1 or MDM4 does not inhibit miR-34a-mediated p53 transcriptional activation.
(A-C) Overexpression of miR-34a resistant SIRT1 (lacking its 3’UTR, immunoblot (B)) does not reduce the miR-34a-mediated increase in the activity of a luciferase reporter driven by a promoter containing 13 p53 consensus binding sites (pG13-luc) (A) or the miR-34a-mediated increase in mRNA of the p53 transcriptional targets CDKN1A, PUMA and TP53INP1, as measured by qRT-PCR (C). (D-F) Same analysis as in (A-C) performed after co-transfecting an HA-tagged MDM4 gene with no 3’UTR and containing synonymous mutations of the miR-34a CDS MREs. All graphs show the mean +/- STDEV of at least three independent experiments (*, p<0.05; **, p<0.01, relative to control miRNA-transfected cells, by 2-tailed Student’s t-test).