Age at Mycobacterium bovis BCG Priming Has Limited Impact on Anti-Tuberculosis Immunity Boosted by Respiratory Mucosal AdHu5Ag85A Immunization in a Murine Model
Fig 3
Memory T cells in the lung and spleen of infant and adult mice following BCG immunization.
Infant and adult mice were BCG immunized and sacrificed at 8 or 16 weeks. Cells from the lung (A) and spleen (B) were stained with extracellular antibodies for memory CD4 T cell markers and analyzed by flow cytometry. Absolute numbers of CD4+CD44+ cells that are Teff/TEM (CD127-/+CD62L-) or TCM (CD127+CD62L+) in the tissues were calculated. Results are from one experiment per timepoint, n = 4/group/timepoint. Data are expressed as Mean ± SEM. *, p < 0.05.