Insights into the Functional Roles of N-Terminal and C-Terminal Domains of Helicobacter pylori DprA
Fig 5
Atomic force microscopy illustrates that DprA forms a highly condensed complex but HpRF forms a less condensed complex with DNA.
Reaction mixture contained 5 nM of circular supercoiled pUC19 DNA and 1 nM DprA or HpRF. Ten microliter aliquots were spotted on fresh mica and visualized as described in materials and methods. (A) Image of supercoiled pUC19 DNA. Image frame containing 1 nM HpDprA (B) or 1 nM HpRF(C) complex with pUC19 DNA (D) Supercoiled pUC19DNA has been linearized with SmaI restriction endonuclease. Complex of linear pUC19DNA with (E) 1 nM HpDprA and (F) 1 nM HpRF.