TrAmplification of Human Dental Follicle Cells by piggyBac Transposon - Mediated Reversible Immortalization System
Fig 8
Chondrogenic differentiation of DFCs, iDFCs and dDFCs.
(A) Induction of Alcian blue in DFCs, iDFCs and dDFCs after chondrogenic induction. Subconfluent DFCs, iDFCs and dDFCs were infected by adenovirus AdBMP9 (a) or AdGFP (data unshown). Alcian blue staining was done after the cells were chondrogenic differentiated for one week (b). (B) The expression of SOX9 after chondrogenic differentiation of DFCs, iDFCs and dDFCs. Subconfluent cells were infected by AdBMP9 (a) or AdGFP. On day 14, cells were lysed, and then subjected to Western blotting using anti- SOX9 antibody. Anti-GAPDH Western blotting ensures equal loading of the samples. Samples infected by AdGFP didn’t express SOX9. (C) Expression of chondrogenic lineage-specific regulators in DFCs, iDFCs and dDFCs stimulated by BMP9. Cells were transfected with AdBMP9 or AdGFP. The assays were done in three experiment. Note: *p < 0.05.