MeCP2 Affects Skeletal Muscle Growth and Morphology through Non Cell-Autonomous Mechanisms
Fig 2
Mecp2-/y muscles exhibit deregulated protein synthesis signalling pathways.
(A) qPCR evaluation of IGF-1 (n = 8) and IGF-R (n = 3) mRNA expression in WT and Mecp2-/y gastrocnemius. All data points were calculated in triplicate, normalized to GAPDH and represented as gene expression relative to WT expression. Significance is calculated with t test (**** P value: <0.0001 for IGF-1; ns, P value: 0.7437 for IGF-R). Data are represented as mean ± s.e.m. (B) ELISA assay quantifying IGF-1 protein levels on WT and Mecp2-/y plasma and triceps (n = 6). All data points were calculated in duplicate. Significance is calculated with t test (*, P value: 0.0313 and **, P value: 0.0096). Data are represented as mean ± s.e.m. (C) Representative WB (left) and summary graph (right) of total rpS6 and phosphorylated rpS6 protein levels in WT and Mecp2-/y triceps lysates (n = 6). GAPDH was included as loading control. Significance is calculated with t test (*, P value: 0.0225; ns, P value: 0.1924). Data are represented as mean ± s.e.m. (D) qPCR evaluation of BDNF mRNA expression in WT and Mecp2-/y Gast. All data points were calculated in triplicate, normalized to GAPDH and represented relative to the WT expression (n = 5). Significance is calculated with t test (*, P value: 0.0425).