Transcriptional Slippage and RNA Editing Increase the Diversity of Transcripts in Chloroplasts: Insight from Deep Sequencing of Vigna radiata Genome and Transcriptome
Fig 3
RNA transcript variation of psbF gene.
(A) CP genome sequence detected by direct sequencing of PCR amplicons. (B) The sequencing “noise” at the U-indel and RNA editing (RE) sites of the transcripts detected by direct sequencing of RT-PCR fragments. Five psbF sequence variants were obtained after sequence analysis of cloned RT-PCR amplicons. One predicted sequence, which could not be detected by sequencing of clones, is marked with a star.