Gastrointestinal Fibroblasts Have Specialized, Diverse Transcriptional Phenotypes: A Comprehensive Gene Expression Analysis of Human Fibroblasts
Fig 3
Anatomical Site Dependent Diversity in Gastrointestinal Fibroblasts.
(A) Unsupervised clustering in the colon, ileum, stomach, duodenum, and esophagus derived fibroblasts samples. Submucosal fibroblasts (SMFs) and subperitoneal fibroblasts (SPFs) from the colon, ileum, and stomach showed different gene expression profiles, whereas those from the duodenum and esophagus were not separated into different clusters. The red bar indicates SMFs samples and the blue bar indicates SPFs samples. The color scale of gene expression is same as Fig 1A. (B) Supervised clustering between SMFs and SPFs samples in the colon, ileum, and stomach. A total of 498 probe sets were selected based on P < 0.05 and fold change > 2.0 (t-test unpaired). The top significant genes are shown on the right. (C) Distinct expression of the genes related with transcriptional regulation, signal ligands, and extracellular matrix remodeling in SMFs and SPFs.