p53 Represses the Oncogenic Sno-MiR-28 Derived from a SnoRNA
Fig 5
sno-miR-28 is over-expressed in breast tumours.
(A) The feed-forward loop between p53, sno-miR-28, and TAF9B is shown as proposed. (B) sno-miR-28, SNORD28, and SNORD25 expression levels were determined using TaqMan assay in breast tumours compared with paired normal adjacent tissues, while SNHG1 expression levels were determined by RT-PCR. In this part of figure, RNA expression levels are shown as the ratio relative to normal tissue expression; e.g., 1 represents equal expression to normal tissues. (C) MCF10A cell proliferation is shown after overexpression of sno-miR-28 compared with negative control RNA (ncRNA). (D) Colony formation assay in MCF10A cells after expression of sno-miR-28 or a negative control RNA (ncRNA). Representative images are included on the left, and relative quantitation of the graph is on the right. ** p<0.01 and * p≤0.1 versus controls for all experiments.