Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish
Fig 8
Visualization of endogenous gene expression by knock-in of the eGFP reporter using the crRNA-tracrRNA-Cas9 protein complex.
(A) Whole-mount in situ hybridization with an epdr1 probe. (B) An uninjected embryo. (C) The embryo injected two crRNAs (epdr1-crRNA; 25 pg + Mbait-crRNA; 25 pg), tracrRNA (100 pg) and Mbait-hs-eGFP (25 pg) with Cas9 protein (400 pg). The expression of eGFP was detected in the anterior central nervous system, including neurons.