Impacts of Autophagy-Inducing Ingredient of Areca Nut on Tumor Cells
Fig 2
Chemical inhibitors of AMPK and CAMKKβ attenuate AIA.
Jurkat T cells treated with ANE 30–100K (30–100K) (9 μg/ml) for 24 hours in the presence or absence of STO-609 (50 μM) (A) or compound C (Com C, 5 μM) (B) were subjected to LC3 and β-actin immunoblotting. Average LC3-II/actin ratio ± SD were plotted as Fig 1A. Cell numbers of Jurkat T cells pretreated with STO-609 (50 μM) (C) or Com C (5 μM) (D) for 2 hours, followed by 30–100K (9 μg/ml) treatment for another day were counted and average fold of untreated control cells ± SD from three independent experiments were plotted. (E) Lysates of Jurkat T cells treated with 30–100K (9 μg/ml) for 24 hours in the presence or absence of the indicated concentrations of AICAR were immunoblotted and LC3-II/actin ratios were presented as (A). (F) Cell numbers of Jurkat T cells treated with the indicated concentrations of 30–100K for 24 hours in the presence or absence of AICAR (250 μM) were determined and average fold of untreated control cells ± SD were plotted. *P < 0.05, **P < 0.01, ***P < 0.001.