Rapid and Specific Enrichment of Culturable Gram Negative Bacteria Using Non-Lethal Copper-Free Click Chemistry Coupled with Magnetic Beads Separation
Fig 2
Kdo-N3 metabolically labels E. coli LPS.
(A) Metabolically incorporated Kdo-N3 in E. coli was revealed by copper-free click chemistry (sulfo-DBCO-biotin followed by an anti-biotin A488 antibody). (B) Frequency distribution of the bacterial fluorescence values in the presence (green bars) or absence of Kdo-N3 (black bars). Scale bar = 1 μm. More than ten independent experiments have been performed and a representative experiment is depicted.