The g.-165 T>C Rather than Methylation Is Associated with Semen Motility in Chinese Holstein Bulls by Regulating the Transcriptional Activity of the HIBADH Gene
Fig 4
Structure of HIBADH, location of the identified SNP (g.-165 T>C), and PCR-RFLP patterns.
Patterns for g.-165 T>C genotypes TT, TC, and CC. M: Marker. Digestion with Sma I of the amplified HIBADH gene g.-165 T>C locus produced fragments of the following sizes: 967 bp for genotype TT; 967, 710, and 257 bp for genotype TC; and 710 and 257 bp for genotype CC.