Nutrient Excess and AMPK Downregulation in Incubated Skeletal Muscle and Muscle of Glucose Infused Rats
Fig 6
Incubation of EDL with 100 μM leucine diminishes AMPK Thr172 phosphorylation, SIRT1 abundance, and NAD/NADH ratio, and increases AMPK Ser485/491 phosphorylation.
EDL muscles were incubated in Krebs-Henseleit solution containing 5.5 mM of glucose with or without 100 μM of leucine for 30, 60 and 120 min. Phosphorylation of AMPK Thr172 (A) and AMPK Ser485/491 (B), SIRT1 protein expression (C) and the NAD/NADH ratio (D) were measured. Results are means + SE (n = 6). *P < 0.05 relative to 30 min incubation with 5.5 mM glucose (without leucine).