Nutrient Excess and AMPK Downregulation in Incubated Skeletal Muscle and Muscle of Glucose Infused Rats
Fig 4
Incubation with elevated glucose levels or leucine or and in vivo glucose infusion increases PP2A activity at 2h (ex vivo) or 8h (in vivo).
EDL muscles incubated for 30, 60 and 120 min in media containing 5.5 or 25 mM glucose (A) or 5.5mM glucose with or without 100μM leucine (C) and red gastrocnemius muscles from rats infused with glucose for 0, 3, 5, or 8h (B) were analyzed for PP2A activity as described in the methods section. Results are means + SE (n = 4–6). *P <0.05 compared to 30 min incubation with 5.5 mM glucose (A) and (C) or the 0h group for infusions (B).