Significance of Relative Position of Cellulases in Designer Cellulosomes for Optimized Cellulolysis
Fig 5
Synergistic activity of all possible combinations of the three T. fusca recombinant enzymes either (i) in the free state, (ii) bound to respective monovalent scaffoldins (CBM-restored) or (iii) bound to different chimaeric scaffoldins, for degradation of Avicel.
Assays were conducted for 72 h, with incubation at 50°C, at an enzymatic concentration of 0.4 μM. Stars indicate a statistically significant difference (lower than) between the couple composed of a-9A and 5A-t in comparison with the combination of the three enzymes together.