A Pilot Study to Assess Effects of Long-Term Inhalation of Airborne Particulate Matter on Early Alzheimer-Like Changes in the Mouse Brain
Fig 3
Brain amyloid precursor protein (APP) levels were reduced after 9 month exposure to airborne particulate matter.
Brains of 9 month exposed filtered air (FA) or PM2.5 (PM) mice were collected with the left hemisphere fixed for immunohistochemistry and the right temporal cortices collected for biochemical analysis. A. Temporal cortex lysates from FA and PM brains were used for western blot analysis using anti-APP, Y188 antibody and α-tubulin (loading control). B. APP optical densities were normalized to their respective loading controls, averaged (+/-SD), and graphed, *p<0.05. C. Brains were immunostained using anti-APP, Y188 antibody. Images shown are representative from the temporal cortex. Arrows indicate regions taken for higher magnification.