In Vitro Recapitulation of Functional Microvessels for the Study of Endothelial Shear Response, Nitric Oxide and [Ca2+]i
Fig 4
The representative immunofluorescent staining confocal images demonstrate the VE-Cadherin distributions of cultured vascular network, a statically cultured endothelial monolayer, and an intact rat mesenteric venule.
A. The schematic image of the network with selected regions as shown in B-D. B-C. VE-Cadherin and cell nuclei staining were shown at the first and third branching regions. D1-D2. VE-Cadherin and cell nuclei staining were shown at the top and bottom surfaces of the second branching regions, respectively. E. VE-cadherin junction distribution of HUVECs cultured under static condition. F. VE-cadherin distribution of endothelial cells from an individually perfused intact rat venule.