Evaluation of Changes in Morphology and Function of Human Induced Pluripotent Stem Cell Derived Cardiomyocytes (HiPSC-CMs) Cultured on an Aligned-Nanofiber Cardiac Patch
Fig 2
Analysis of gap junctions in hiPSC-CMs by confocal microscopy in cells seeded on flat plate and aligned nanofiber.
The gap junctions in hiPSC-CMs was evaluated by CX-43 immunostaining of human cardiomyocytes seeded on flat surface versus aligned-nanofiber coated coverslips. A) Flat plate, CX-43 (Red), SAA (Green) and DAPI (Blue); B) Aligned nanofiber, CX-43 (Red), SAA (Green) and DAPI (Blue); C) Quantification of gap junctions (% CX-43 positive area fraction per cell) in hiPSC-CMs seeded on flat plate and aligned nanofiber. There were no significant differences between the two groups. All values expressed as mean ± SD (n = 4/group).