Adducin Is Involved in Endothelial Barrier Stabilization
Fig 1
α- and γ-adducins are expressed in microvascular endothelium and α-adducin is colocalized with junctional proteins.
(A) Protein abundance of α- and γ-adducins was evaluated by Western blot. α- tubulin or ß-actin were used to control for equal gel loading. (B) Expression profile of both adducin isoforms in bar graph representing the signal intensity analyzed by densitometry and normalized to the protein level of the respective loading control (Means ± SEM). In comparison to MyEnd cells, HDMEC cells showed significantly higher expression of α- and γ-adducins in total cell lysates (*P<0.05, **P<0.01, paired t-test, 2-tails) (C-F) Dual immunostaining for α-adducin with either AJ or TJ proteins in HDMEC and MyEnd cells revealed colocalization of α-adducin with VE-cadherin (AJ) and claudin-5 (TJ), respectively. The results are representative of three or more independent experiments. Scale bar = 20 μm.