(6E,10E) Isopolycerasoidol and (6E,10E) Isopolycerasoidol Methyl Ester, Prenylated Benzopyran Derivatives from Pseuduvaria monticola Induce Mitochondrial-Mediated Apoptosis in Human Breast Adenocarcinoma Cells
Fig 3
Compound (1) and (2) induce apoptosis rather than senescence in breast cancer cells.
(A and B) MCF-7, MDA-MB-231 and MCF-10A cells were stained with Annexin V/PI and subjected to flow cytometry analysis. The four quadrants represent living cells (Annexin V-PI-), early apoptotic (Annexin V+PI-), late apoptosis (Annexin+PI+) or necrotic (Annexin V-PI+) stages. Values shown were percentages of each quadrant. *P<0.05, in comparison to control. (C) MCF-7 and MDA-MB-231 cells were treated with DMSO (solvent), (1) or (2) for 24 hours before subjected to in situ senescence-associated β-gal staining at pH 6. The development of blue color was examined by bright field microscopy. (Magnification 200X). Right panel: senescent cancer-associated fibroblast strain, BICR78F was included as a positive control for senescence-associated β-gal staining.