Catch Muscle Myorod Modulates ATPase Activity of Myosin in a Phosphorylation-Dependent Way
Fig 4
Superprecipitation and clearing of reconstituted actomyosins in the presence of CF1 (enriched with inactivated myosin and twitchin) and CF3 (pure myorod).
A. AM, actomyosin (control); AM+CF1 (effect of myosin and twitchin contaminates). B. AM-PhosMR, co-filaments of myosin / phosphorylated myorod; AM-UnPhosMR, co-filaments of myosin / non-phosphorylated myorod. Concentration used: F-actin, 0.2 mg/ml; myosin, 0.1 mg/ml; myorod, 0.1 mg/ml.