A 1-Cys Peroxiredoxin from a Thermophilic Archaeon Moonlights as a Molecular Chaperone to Protect Protein and DNA against Stress-Induced Damage
Fig 4
Structural analysis of 1-Cys TkPrx mutants.
(A) Wild type and mutants were analysed using a Superdex 200 10/300 GL column (left) and 8% BN-PAGE (right). The variant lines indicate the different mutants of 1-Cys TkPrx: Wild type (solid line, blue), mutant C46S (dotted line, red), mutant C205S (long dashed line, magenta) and mutant C211S (long dashed line, black). (B) Appearance of the negatively stained wild type and mutant proteins. Average images of C46S, C205S, and C211S produced from 1205, 122, and 176 images, respectively. The scale bar indicates 50 nm, and the magnification is ×42,000. (C) Modelling of the top view based on image processing of the C46S mutant protein of 1-Cys TkPrx.