Actin Inhibition Increases Megakaryocyte Proplatelet Formation through an Apoptosis-Dependent Mechanism
Fig 1
Actin polymerization inhibitor (Latrunculin) increases megakaryocyte maturation and proplatelet formation.
A,B) FACS profiles of propidium iodide staining indicating ploidy of control (A) and latrunculin treated (B) cells. C) Actin inhibition increases the levels of polyploid megakaryocytes in culture. Y axis represents the % of cells with ploidy ≥8N. D,E) Wright-Giemsa stained megakaryocytes showing increased polyploidization with actin-inhibitor treatement: D) Untreated control E) Actin polymerization inhibitor treated. F) Actin inhibition increases the levels of megakaryocytes proplatelet formation in culture. Y-axis represents the sum of all proplatelet (PP) segments counted inside of grids in 10 random field images (40x magnification). G,H) Light microscopy images showing increased proplatelet formation after actin polymerization inhibitor treatment: G) Untreated control H) Actin polymerization inhibitor treated. * p<0.05