Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
Fig 5
No detectable pAKT-ir in ICC of WT, Spry4 KO and KitWT/K641E antrum.
Widefield microscopy, sequential channels acquisitions. Left column: PDE3A immunoreactivity (-ir) ICC in WT, Spry4 KO and KitWT/K641E. Middle column: pAKT-ir in the 3 genotypes. Right column: merged images: PDE3A-ir and pAKT displayed in green and in red, respectively. pAKT (red) was consistently detected in myenteric plexus and nerve fibers in the muscularis propria but not in PDE3A-ir ICC (green). Abbreviations: LM: longitudinal muscle layer, CM: circular muscle layer, *: myenteric plexus, scale bar: 100μm.